cd138 magnetic beads  (Miltenyi Biotec)

Journal: medRxiv

Article Title: Single-Cell Analysis Reveals Inflammatory–Immunosuppressive Niches in Daratumumab-Resistant Primary AL Amyloidosis

doi: 10.64898/2026.03.28.26349317

Figure Lengend Snippet: (A) UMAP of BM PCs and microenvironment cells in the whole BM samples. (B) PC–centered immune cell communication algorithm to compute the hematologic response-related ligand-receptor cell-cell communication probabilities. (C) Dot plot showing response-related ligand–receptor interactions between PCs and microenvironment immune cells grouped by pre/post-treatment and hematologic response. (D) HLA-E–KLRK1 signaling representing non-classical MHC class I–mediated ligand–receptor pairs associated with hematologic response. (E) Prostaglandin pathway–mediated ligand–receptor pairs associated with hematologic response. (F) Multiplex immunofluorescence validation of prostaglandin pathway activation in BM tissue sections. Colors: DAPI (blue), CD138 (red), CD14 (green), PTGS2 (yellow), PTGER2/4 (cyan), PTGES3 (white), PTGES2 (orange). Scale bars: column 1-4 (20 μm); column 5 (100 μm).

Article Snippet: PC enrichment was performed using CD138 + Magnetic Beads (Miltenyi Biotec, Bergisch Gladbach, Germany; Cat# 130-051-301), MS columns (Cat# 130-042-201), and a MiniMACS separator (Cat# 130-042-102).

Techniques: Multiplex Assay, Immunofluorescence, Biomarker Discovery, Activation Assay

Journal: International Journal of Oncology

Article Title: LGR4 promotes proliferation and homing via activation of the NF-κB signaling pathway in multiple myeloma

doi: 10.3892/ijo.2025.5718

Figure Lengend Snippet: High expression of LGR4 is associated with cell adhesion and poor prognosis in multiple myeloma. (A) Gene expression heatmap of LGR4 (Red) and Wnt/β-catenin signal related genes (Black), Cell adhesion associated genes (Blue) in CD138 + cells from HD (n=22), MGUS (n=44) and MM (n=351). (B) Pearson's correlation analysis of the relationship between LGR4 and cell adhesion genes. Red for positive, blue for negative. (C) Gene expression heatmap of OCI-My5 and OCI-T3 rd -luc with LGR4 (Red), Wnt/β-catenin signal genes (Black), Cell adhesion associated genes (Blue). (D) Gene expression heatmap of KMS28-BM and KMS28-PE with LGR4 (Red), Wnt/β-catenin signal genes (Black), Cell adhesion associated genes (Blue). (E) Representative images of immunofluorescence images of LGR4 and ZEB1 protein expression in KMS28-BM and KMS28-PE. Scale bars, 50 μ m. (F) Kaplan-Meier analyses of overall survival in MM patients with LGR4 low ZEB1 low (n=58), LGR4 low ZEB1 high (n=7), LGR4 high ZEB1 low (n=309) and LGR4 high ZEB1 high (n=85) from GSE2658. HD, healthy donors; MGUS, monoclonal gammopathy of undetermined significance; MM, multiple myeloma; BM, bone marrow; PE, pleural effusion; ZEB1, Zinc Finger E-Box Binding Homeobox 1.

Article Snippet: CD138 + plasma cells were isolated by using anti-human CD138 magnetic beads (Miltenyi Biotec GmbH) and incubated in 4°C for 15 min with monocytes isolated from BM samples using lymphocyte separation medium (cat. no. LTS1077; TBD; https://www.tbdscience.com/ ).

Techniques: Expressing, Gene Expression, Immunofluorescence, Binding Assay

Journal: International Journal of Oncology

Article Title: LGR4 promotes proliferation and homing via activation of the NF-κB signaling pathway in multiple myeloma

doi: 10.3892/ijo.2025.5718

Figure Lengend Snippet: Overexpression of LGR4 promotes cells' homing to the BM and MM progression in vivo . (A) Schematic of in vivo experiments. (B) Tumor-associated live imaging of NCG mice injected with OCI-Ctrl or OCI-LGR4-OE cells at 4 and 6 weeks (n=5 for each group). (C) Quantification of luminescence intensity in live NCG mice. (D) Flow cytometric analysis images and statistics of the human MM cell proportion in the bone marrow after sacrificing NCG mice. (E) Micro-CT images of tibia derived from NCG mice. (F) Quantification of bone microstructural parameters, namely BV/TV, Tb.N, Tb.Th and Tb.Sp (n=3). (G) TRAP staining for NCG xenografted mice bone marrow section. Scale bars, 200, 25 and 10 μ m. (I) The quantification of the number of Trap-positive osteoclast cells is presented in the column graph. (H) Neoplastic CD138-positive plasma cells. Scale bars, 10 μ m. (J) The quantification of the number of neoplastic CD138 positive plasma cells is presented in the column graph. Statistical analyses were performed using Student's t-test. * P<0.05, ** P<0.01, *** P<0.001 and ****P<0.0001. BM, bone marrow; MM, multiple myeloma; LGR4-OE, LGR4 overexpression; BV/TV, trabecular bone volume fraction; Tb.N, trabecular number; Tb.Th, trabecular thickness; Tb.Sp, trabecular separation; TRAP, tartrate-resistant acid phosphatase; EV, empty vector; ns, not significant (P>0.05).

Article Snippet: CD138 + plasma cells were isolated by using anti-human CD138 magnetic beads (Miltenyi Biotec GmbH) and incubated in 4°C for 15 min with monocytes isolated from BM samples using lymphocyte separation medium (cat. no. LTS1077; TBD; https://www.tbdscience.com/ ).

Techniques: Over Expression, In Vivo, Imaging, Injection, Micro-CT, Derivative Assay, Staining, Clinical Proteomics, Plasmid Preparation